episomal vector Search Results


stemcircle episomal vector  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc stemcircle episomal vector
Stemcircle Episomal Vector, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/episomal+vector/pm24740884-50-0-3?v=STEMCELL+Technologies+Inc
Average 90 stars, based on 1 article reviews
stemcircle episomal vector - by Bioz Stars, 2026-07
90/100 stars
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episomal vectors  (Lonza)
90
Lonza episomal vectors
Episomal Vectors, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/episomal+vector/pm34339420-43-5-12?v=Lonza
Average 90 stars, based on 1 article reviews
episomal vectors - by Bioz Stars, 2026-07
90/100 stars
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plasmid dna of episomal vectors  (Lonza)
90
Lonza plasmid dna of episomal vectors
Introduction of the GFP reporter to the BCL-XL vector helped to monitor <t>nucleofection</t> efficiency and identify fully reprogrammed clones. (A): Diagram of newly constructed GBX vector. (B): GFP expression was used to monitor nucleofection efficiency at day 2 after nucleofection. The microphotograph was taken at 10× magnification. (C): Reprogrammed cells were analyzed at day 13 using flow cytometric analysis. Many cells that gained TRA-1-60 tended to lose GFP expression. (D): Imaging analysis showed that fully reprogrammed iPS-like colonies that gained TRA-1-60 expression (stained in red) lacked the GFP signal (arrow). Scale bar = 200 μm. (E): The GFP-expressing GBX plasmid has potency similar to that of the MBX plasmid expressing BCL-XL alone in reprogramming adult PB MNCs in combination with two other episomal vectors (MOS and MMK). Abbreviations: EBNA1, Epstein-Barr nuclear antigen 1; eGFP, enhanced green fluorescent protein; GBX, modified vector expressing GFP and BCL-XL; GFP, green fluorescent protein; iPS, induced pluripotent stem cells; M, million; MBX, modified vector expressing BCL-XL; MNCs, mononuclear cells; NS, no significant differences on statistical analysis; PB, peripheral blood; SFFV, spleen focus-forming virus; SSC, side scatter.
Plasmid Dna Of Episomal Vectors, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/episomal+vector/pmc04367508-140-34-38?v=Lonza
Average 90 stars, based on 1 article reviews
plasmid dna of episomal vectors - by Bioz Stars, 2026-07
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episomal vectors (contains mir-302b, c, a, d and mir-367)  (Lonza)
90
Lonza episomal vectors (contains mir-302b, c, a, d and mir-367)
Introduction of the GFP reporter to the BCL-XL vector helped to monitor <t>nucleofection</t> efficiency and identify fully reprogrammed clones. (A): Diagram of newly constructed GBX vector. (B): GFP expression was used to monitor nucleofection efficiency at day 2 after nucleofection. The microphotograph was taken at 10× magnification. (C): Reprogrammed cells were analyzed at day 13 using flow cytometric analysis. Many cells that gained TRA-1-60 tended to lose GFP expression. (D): Imaging analysis showed that fully reprogrammed iPS-like colonies that gained TRA-1-60 expression (stained in red) lacked the GFP signal (arrow). Scale bar = 200 μm. (E): The GFP-expressing GBX plasmid has potency similar to that of the MBX plasmid expressing BCL-XL alone in reprogramming adult PB MNCs in combination with two other episomal vectors (MOS and MMK). Abbreviations: EBNA1, Epstein-Barr nuclear antigen 1; eGFP, enhanced green fluorescent protein; GBX, modified vector expressing GFP and BCL-XL; GFP, green fluorescent protein; iPS, induced pluripotent stem cells; M, million; MBX, modified vector expressing BCL-XL; MNCs, mononuclear cells; NS, no significant differences on statistical analysis; PB, peripheral blood; SFFV, spleen focus-forming virus; SSC, side scatter.
Episomal Vectors (Contains Mir 302b, C, A, D And Mir 367), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/episomal+vector/pmc04682290__mmc1-39-48-59?v=Lonza
Average 90 stars, based on 1 article reviews
episomal vectors (contains mir-302b, c, a, d and mir-367) - by Bioz Stars, 2026-07
90/100 stars
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ige orip/ebna-1-based episomal mammalian expression vector poe  (MedImmune llc)
90
MedImmune llc ige orip/ebna-1-based episomal mammalian expression vector poe
Introduction of the GFP reporter to the BCL-XL vector helped to monitor <t>nucleofection</t> efficiency and identify fully reprogrammed clones. (A): Diagram of newly constructed GBX vector. (B): GFP expression was used to monitor nucleofection efficiency at day 2 after nucleofection. The microphotograph was taken at 10× magnification. (C): Reprogrammed cells were analyzed at day 13 using flow cytometric analysis. Many cells that gained TRA-1-60 tended to lose GFP expression. (D): Imaging analysis showed that fully reprogrammed iPS-like colonies that gained TRA-1-60 expression (stained in red) lacked the GFP signal (arrow). Scale bar = 200 μm. (E): The GFP-expressing GBX plasmid has potency similar to that of the MBX plasmid expressing BCL-XL alone in reprogramming adult PB MNCs in combination with two other episomal vectors (MOS and MMK). Abbreviations: EBNA1, Epstein-Barr nuclear antigen 1; eGFP, enhanced green fluorescent protein; GBX, modified vector expressing GFP and BCL-XL; GFP, green fluorescent protein; iPS, induced pluripotent stem cells; M, million; MBX, modified vector expressing BCL-XL; MNCs, mononuclear cells; NS, no significant differences on statistical analysis; PB, peripheral blood; SFFV, spleen focus-forming virus; SSC, side scatter.
Ige Orip/Ebna 1 Based Episomal Mammalian Expression Vector Poe, supplied by MedImmune llc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/episomal+vector/pmc04718782-159-30-37?v=MedImmune+llc
Average 90 stars, based on 1 article reviews
ige orip/ebna-1-based episomal mammalian expression vector poe - by Bioz Stars, 2026-07
90/100 stars
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episomal expression vectors pcep-pu-strep ii-tags  (SLIT2 LTD)
90
SLIT2 LTD episomal expression vectors pcep-pu-strep ii-tags
Introduction of the GFP reporter to the BCL-XL vector helped to monitor <t>nucleofection</t> efficiency and identify fully reprogrammed clones. (A): Diagram of newly constructed GBX vector. (B): GFP expression was used to monitor nucleofection efficiency at day 2 after nucleofection. The microphotograph was taken at 10× magnification. (C): Reprogrammed cells were analyzed at day 13 using flow cytometric analysis. Many cells that gained TRA-1-60 tended to lose GFP expression. (D): Imaging analysis showed that fully reprogrammed iPS-like colonies that gained TRA-1-60 expression (stained in red) lacked the GFP signal (arrow). Scale bar = 200 μm. (E): The GFP-expressing GBX plasmid has potency similar to that of the MBX plasmid expressing BCL-XL alone in reprogramming adult PB MNCs in combination with two other episomal vectors (MOS and MMK). Abbreviations: EBNA1, Epstein-Barr nuclear antigen 1; eGFP, enhanced green fluorescent protein; GBX, modified vector expressing GFP and BCL-XL; GFP, green fluorescent protein; iPS, induced pluripotent stem cells; M, million; MBX, modified vector expressing BCL-XL; MNCs, mononuclear cells; NS, no significant differences on statistical analysis; PB, peripheral blood; SFFV, spleen focus-forming virus; SSC, side scatter.
Episomal Expression Vectors Pcep Pu Strep Ii Tags, supplied by SLIT2 LTD, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/episomal+vector/pm36792623-395-12-18?v=SLIT2+LTD
Average 90 stars, based on 1 article reviews
episomal expression vectors pcep-pu-strep ii-tags - by Bioz Stars, 2026-07
90/100 stars
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pebmulti-ble-egfp episomal vector  (FUJIFILM)
90
FUJIFILM pebmulti-ble-egfp episomal vector
Introduction of the GFP reporter to the BCL-XL vector helped to monitor <t>nucleofection</t> efficiency and identify fully reprogrammed clones. (A): Diagram of newly constructed GBX vector. (B): GFP expression was used to monitor nucleofection efficiency at day 2 after nucleofection. The microphotograph was taken at 10× magnification. (C): Reprogrammed cells were analyzed at day 13 using flow cytometric analysis. Many cells that gained TRA-1-60 tended to lose GFP expression. (D): Imaging analysis showed that fully reprogrammed iPS-like colonies that gained TRA-1-60 expression (stained in red) lacked the GFP signal (arrow). Scale bar = 200 μm. (E): The GFP-expressing GBX plasmid has potency similar to that of the MBX plasmid expressing BCL-XL alone in reprogramming adult PB MNCs in combination with two other episomal vectors (MOS and MMK). Abbreviations: EBNA1, Epstein-Barr nuclear antigen 1; eGFP, enhanced green fluorescent protein; GBX, modified vector expressing GFP and BCL-XL; GFP, green fluorescent protein; iPS, induced pluripotent stem cells; M, million; MBX, modified vector expressing BCL-XL; MNCs, mononuclear cells; NS, no significant differences on statistical analysis; PB, peripheral blood; SFFV, spleen focus-forming virus; SSC, side scatter.
Pebmulti Ble Egfp Episomal Vector, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/episomal+vector/pm27927754-212-14-17?v=FUJIFILM
Average 90 stars, based on 1 article reviews
pebmulti-ble-egfp episomal vector - by Bioz Stars, 2026-07
90/100 stars
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episomal ipsc reprogramming vectors  (Lonza)
90
Lonza episomal ipsc reprogramming vectors
Introduction of the GFP reporter to the BCL-XL vector helped to monitor <t>nucleofection</t> efficiency and identify fully reprogrammed clones. (A): Diagram of newly constructed GBX vector. (B): GFP expression was used to monitor nucleofection efficiency at day 2 after nucleofection. The microphotograph was taken at 10× magnification. (C): Reprogrammed cells were analyzed at day 13 using flow cytometric analysis. Many cells that gained TRA-1-60 tended to lose GFP expression. (D): Imaging analysis showed that fully reprogrammed iPS-like colonies that gained TRA-1-60 expression (stained in red) lacked the GFP signal (arrow). Scale bar = 200 μm. (E): The GFP-expressing GBX plasmid has potency similar to that of the MBX plasmid expressing BCL-XL alone in reprogramming adult PB MNCs in combination with two other episomal vectors (MOS and MMK). Abbreviations: EBNA1, Epstein-Barr nuclear antigen 1; eGFP, enhanced green fluorescent protein; GBX, modified vector expressing GFP and BCL-XL; GFP, green fluorescent protein; iPS, induced pluripotent stem cells; M, million; MBX, modified vector expressing BCL-XL; MNCs, mononuclear cells; NS, no significant differences on statistical analysis; PB, peripheral blood; SFFV, spleen focus-forming virus; SSC, side scatter.
Episomal Ipsc Reprogramming Vectors, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/episomal+vector/pm37343430-34-13-25?v=Lonza
Average 90 stars, based on 1 article reviews
episomal ipsc reprogramming vectors - by Bioz Stars, 2026-07
90/100 stars
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episomal vectors  (XCell Science)
90
XCell Science episomal vectors
Introduction of the GFP reporter to the BCL-XL vector helped to monitor <t>nucleofection</t> efficiency and identify fully reprogrammed clones. (A): Diagram of newly constructed GBX vector. (B): GFP expression was used to monitor nucleofection efficiency at day 2 after nucleofection. The microphotograph was taken at 10× magnification. (C): Reprogrammed cells were analyzed at day 13 using flow cytometric analysis. Many cells that gained TRA-1-60 tended to lose GFP expression. (D): Imaging analysis showed that fully reprogrammed iPS-like colonies that gained TRA-1-60 expression (stained in red) lacked the GFP signal (arrow). Scale bar = 200 μm. (E): The GFP-expressing GBX plasmid has potency similar to that of the MBX plasmid expressing BCL-XL alone in reprogramming adult PB MNCs in combination with two other episomal vectors (MOS and MMK). Abbreviations: EBNA1, Epstein-Barr nuclear antigen 1; eGFP, enhanced green fluorescent protein; GBX, modified vector expressing GFP and BCL-XL; GFP, green fluorescent protein; iPS, induced pluripotent stem cells; M, million; MBX, modified vector expressing BCL-XL; MNCs, mononuclear cells; NS, no significant differences on statistical analysis; PB, peripheral blood; SFFV, spleen focus-forming virus; SSC, side scatter.
Episomal Vectors, supplied by XCell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/episomal+vector/pm38225227-200-15-17?v=XCell+Science
Average 90 stars, based on 1 article reviews
episomal vectors - by Bioz Stars, 2026-07
90/100 stars
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episomal vectors encoding four reprogramming factors  (Applied StemCell Inc)
90
Applied StemCell Inc episomal vectors encoding four reprogramming factors
Introduction of the GFP reporter to the BCL-XL vector helped to monitor <t>nucleofection</t> efficiency and identify fully reprogrammed clones. (A): Diagram of newly constructed GBX vector. (B): GFP expression was used to monitor nucleofection efficiency at day 2 after nucleofection. The microphotograph was taken at 10× magnification. (C): Reprogrammed cells were analyzed at day 13 using flow cytometric analysis. Many cells that gained TRA-1-60 tended to lose GFP expression. (D): Imaging analysis showed that fully reprogrammed iPS-like colonies that gained TRA-1-60 expression (stained in red) lacked the GFP signal (arrow). Scale bar = 200 μm. (E): The GFP-expressing GBX plasmid has potency similar to that of the MBX plasmid expressing BCL-XL alone in reprogramming adult PB MNCs in combination with two other episomal vectors (MOS and MMK). Abbreviations: EBNA1, Epstein-Barr nuclear antigen 1; eGFP, enhanced green fluorescent protein; GBX, modified vector expressing GFP and BCL-XL; GFP, green fluorescent protein; iPS, induced pluripotent stem cells; M, million; MBX, modified vector expressing BCL-XL; MNCs, mononuclear cells; NS, no significant differences on statistical analysis; PB, peripheral blood; SFFV, spleen focus-forming virus; SSC, side scatter.
Episomal Vectors Encoding Four Reprogramming Factors, supplied by Applied StemCell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/episomal+vector/10__1194_slash_jlr__m081323-44-17-30?v=Applied+StemCell+Inc
Average 90 stars, based on 1 article reviews
episomal vectors encoding four reprogramming factors - by Bioz Stars, 2026-07
90/100 stars
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episomal vectors  (FUJIFILM)
90
FUJIFILM episomal vectors
Introduction of the GFP reporter to the BCL-XL vector helped to monitor <t>nucleofection</t> efficiency and identify fully reprogrammed clones. (A): Diagram of newly constructed GBX vector. (B): GFP expression was used to monitor nucleofection efficiency at day 2 after nucleofection. The microphotograph was taken at 10× magnification. (C): Reprogrammed cells were analyzed at day 13 using flow cytometric analysis. Many cells that gained TRA-1-60 tended to lose GFP expression. (D): Imaging analysis showed that fully reprogrammed iPS-like colonies that gained TRA-1-60 expression (stained in red) lacked the GFP signal (arrow). Scale bar = 200 μm. (E): The GFP-expressing GBX plasmid has potency similar to that of the MBX plasmid expressing BCL-XL alone in reprogramming adult PB MNCs in combination with two other episomal vectors (MOS and MMK). Abbreviations: EBNA1, Epstein-Barr nuclear antigen 1; eGFP, enhanced green fluorescent protein; GBX, modified vector expressing GFP and BCL-XL; GFP, green fluorescent protein; iPS, induced pluripotent stem cells; M, million; MBX, modified vector expressing BCL-XL; MNCs, mononuclear cells; NS, no significant differences on statistical analysis; PB, peripheral blood; SFFV, spleen focus-forming virus; SSC, side scatter.
Episomal Vectors, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/episomal+vector/pmc06819403-2-12-0?v=FUJIFILM
Average 90 stars, based on 1 article reviews
episomal vectors - by Bioz Stars, 2026-07
90/100 stars
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episomal vectors stemcircles -lgnso  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc episomal vectors stemcircles -lgnso
Introduction of the GFP reporter to the BCL-XL vector helped to monitor <t>nucleofection</t> efficiency and identify fully reprogrammed clones. (A): Diagram of newly constructed GBX vector. (B): GFP expression was used to monitor nucleofection efficiency at day 2 after nucleofection. The microphotograph was taken at 10× magnification. (C): Reprogrammed cells were analyzed at day 13 using flow cytometric analysis. Many cells that gained TRA-1-60 tended to lose GFP expression. (D): Imaging analysis showed that fully reprogrammed iPS-like colonies that gained TRA-1-60 expression (stained in red) lacked the GFP signal (arrow). Scale bar = 200 μm. (E): The GFP-expressing GBX plasmid has potency similar to that of the MBX plasmid expressing BCL-XL alone in reprogramming adult PB MNCs in combination with two other episomal vectors (MOS and MMK). Abbreviations: EBNA1, Epstein-Barr nuclear antigen 1; eGFP, enhanced green fluorescent protein; GBX, modified vector expressing GFP and BCL-XL; GFP, green fluorescent protein; iPS, induced pluripotent stem cells; M, million; MBX, modified vector expressing BCL-XL; MNCs, mononuclear cells; NS, no significant differences on statistical analysis; PB, peripheral blood; SFFV, spleen focus-forming virus; SSC, side scatter.
Episomal Vectors Stemcircles Lgnso, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/episomal+vector/pm23256541-120-10-15?v=STEMCELL+Technologies+Inc
Average 90 stars, based on 1 article reviews
episomal vectors stemcircles -lgnso - by Bioz Stars, 2026-07
90/100 stars
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Image Search Results


Introduction of the GFP reporter to the BCL-XL vector helped to monitor nucleofection efficiency and identify fully reprogrammed clones. (A): Diagram of newly constructed GBX vector. (B): GFP expression was used to monitor nucleofection efficiency at day 2 after nucleofection. The microphotograph was taken at 10× magnification. (C): Reprogrammed cells were analyzed at day 13 using flow cytometric analysis. Many cells that gained TRA-1-60 tended to lose GFP expression. (D): Imaging analysis showed that fully reprogrammed iPS-like colonies that gained TRA-1-60 expression (stained in red) lacked the GFP signal (arrow). Scale bar = 200 μm. (E): The GFP-expressing GBX plasmid has potency similar to that of the MBX plasmid expressing BCL-XL alone in reprogramming adult PB MNCs in combination with two other episomal vectors (MOS and MMK). Abbreviations: EBNA1, Epstein-Barr nuclear antigen 1; eGFP, enhanced green fluorescent protein; GBX, modified vector expressing GFP and BCL-XL; GFP, green fluorescent protein; iPS, induced pluripotent stem cells; M, million; MBX, modified vector expressing BCL-XL; MNCs, mononuclear cells; NS, no significant differences on statistical analysis; PB, peripheral blood; SFFV, spleen focus-forming virus; SSC, side scatter.

Journal: Stem Cells Translational Medicine

Article Title: A Facile Method to Establish Human Induced Pluripotent Stem Cells From Adult Blood Cells Under Feeder-Free and Xeno-Free Culture Conditions: A Clinically Compliant Approach

doi: 10.5966/sctm.2014-0214

Figure Lengend Snippet: Introduction of the GFP reporter to the BCL-XL vector helped to monitor nucleofection efficiency and identify fully reprogrammed clones. (A): Diagram of newly constructed GBX vector. (B): GFP expression was used to monitor nucleofection efficiency at day 2 after nucleofection. The microphotograph was taken at 10× magnification. (C): Reprogrammed cells were analyzed at day 13 using flow cytometric analysis. Many cells that gained TRA-1-60 tended to lose GFP expression. (D): Imaging analysis showed that fully reprogrammed iPS-like colonies that gained TRA-1-60 expression (stained in red) lacked the GFP signal (arrow). Scale bar = 200 μm. (E): The GFP-expressing GBX plasmid has potency similar to that of the MBX plasmid expressing BCL-XL alone in reprogramming adult PB MNCs in combination with two other episomal vectors (MOS and MMK). Abbreviations: EBNA1, Epstein-Barr nuclear antigen 1; eGFP, enhanced green fluorescent protein; GBX, modified vector expressing GFP and BCL-XL; GFP, green fluorescent protein; iPS, induced pluripotent stem cells; M, million; MBX, modified vector expressing BCL-XL; MNCs, mononuclear cells; NS, no significant differences on statistical analysis; PB, peripheral blood; SFFV, spleen focus-forming virus; SSC, side scatter.

Article Snippet: To test new EBNA1/OriP episomal vectors compared with the pEB-C5 and pEB-Tg combination or Okita-Yamanaka combination, 2 × 10 6 cultured erythroblasts were mixed with a total of 10 μg of plasmid DNA of episomal vectors for nucleofection (Lonza, Walkersville, MD, http://www.lonza.com ).

Techniques: Plasmid Preparation, Clone Assay, Construct, Expressing, Imaging, Staining, Modification, Virus

Replacement of MEFs by vitronectin as a cell culture substrate maintained high reprogramming efficiency with the improved vector combination. (A): Schematic diagram of using VTN to substitute for MEFs as the coating material for tissue plates used at day 2 after nucleofection. A chemically defined, xeno-free E8 medium was used to replace the human ES cell medium that has animal products in the serum replacement. (B): VTN was shown to sustain high reprogramming efficiency under the MOS/MMK/MBX (pEV) vector combination with six different samples of adult PB MNCs of randomly selected donors. Abbreviations: BSA, bovine serum albumin; DMEM, Dulbecco’s modified Eagle’s medium; ES, embryonic stem (cell); FBS, fetal bovine serum; M, million; MEF, mouse embryonic fibroblast; MNCs, mononuclear cells; NaB, sodium butyrate; PB, peripheral blood; SFM, serum-free medium; VTN, vitronectin.

Journal: Stem Cells Translational Medicine

Article Title: A Facile Method to Establish Human Induced Pluripotent Stem Cells From Adult Blood Cells Under Feeder-Free and Xeno-Free Culture Conditions: A Clinically Compliant Approach

doi: 10.5966/sctm.2014-0214

Figure Lengend Snippet: Replacement of MEFs by vitronectin as a cell culture substrate maintained high reprogramming efficiency with the improved vector combination. (A): Schematic diagram of using VTN to substitute for MEFs as the coating material for tissue plates used at day 2 after nucleofection. A chemically defined, xeno-free E8 medium was used to replace the human ES cell medium that has animal products in the serum replacement. (B): VTN was shown to sustain high reprogramming efficiency under the MOS/MMK/MBX (pEV) vector combination with six different samples of adult PB MNCs of randomly selected donors. Abbreviations: BSA, bovine serum albumin; DMEM, Dulbecco’s modified Eagle’s medium; ES, embryonic stem (cell); FBS, fetal bovine serum; M, million; MEF, mouse embryonic fibroblast; MNCs, mononuclear cells; NaB, sodium butyrate; PB, peripheral blood; SFM, serum-free medium; VTN, vitronectin.

Article Snippet: To test new EBNA1/OriP episomal vectors compared with the pEB-C5 and pEB-Tg combination or Okita-Yamanaka combination, 2 × 10 6 cultured erythroblasts were mixed with a total of 10 μg of plasmid DNA of episomal vectors for nucleofection (Lonza, Walkersville, MD, http://www.lonza.com ).

Techniques: Cell Culture, Plasmid Preparation, Modification